The study focused specifically on eye tissues, which are damaged by scarring in diabetic patients. This technique is extremely effective at giving us the data we need on these tissues,” says Dr. Albena Ivanisevic, co-author of a paper who is an associate professor of materials science and engineering at NC State and associate professor of the joint biomedical engineering program at NC State and the University of North Carolina at Chapel Hill.
“Specifically, it gives a great deal of information on the composition of these tissues, as well as the tissue’s topography, or surface characteristics” they added.
There are multiple treatments for scar tissue formation. In the United States, a common technique is for a surgeon to peel off the inner limiting membrane (ILM), removing the scar tissue with it. In many other parts of the world, surgeons inject dye into the eye to better distinguish the parts of the eye they will operate on.
(This process is not currently allowed in the United States, due to concerns about the dye’s toxicity)
The researchers launched a project to determine if bimodal dual AC mode microscopy could be used to provide a better understanding of the topographical properties of the ILM. Further, they wanted to use the technology to see if it offered insight into how—or whether—various dyes affect the topographical characteristics of the ILM. “All of this information could be used to improve surgical outcomes and to foster research into additional treatments for the condition,” Ivanisevic says.
The researchers found that bimodal dual AC mode microscopy, an atomic force imaging technique, captured the properties of the tissue in exceptional detail. Atomic force imaging effectively runs a probe over the surface of a material to collect data on its topography, similar to the way in which a record player’s needle runs over the surface of an album.
“The next step would be to use this technology to assess the utility—and potential risk—of various dyes,” Ivanisevic says. “If we can find a dye that is extremely effective and poses little risk, it may be approved for use in future surgeries.”