Advantages of Multiplex polymerase chain reaction (PCR) pathogen detection


This study was conducted by Tran, at UC Davis and this study is to determine the clinical value of multiplex polymerase chain reaction (PCR) study for enhancing pathogen detection in patients with suspected septicemia after trauma, emergency, and burn surgery.

Study suggests that PCR-based pathogen detection quickly reveals occult blood stream infections in these high-risk patients and may accelerate the initiation of targeted antimicrobial therapy.

Though results warrant further investigation through interventional trials, the following are the noteworthy as far multiplex PCR is concerned:

  • PCR detected the presence of pathogens more frequently than blood culture
  • Faster results were reported compared to blood culture results.
  • Severity scores were significantly greater in PCR-positive trauma and emergency surgery patients.
  • The lack of pathogen DNA as determined by PCR served as a significant predictor of survival in the combined patient population.
  • PCR testing independent of traditional prompts for culturing may have clinical value in burn patients.

Seen first at PI posted by Dr. Larry H Bernstein, MD, FCAP : Molecular pathogen identification comes to the bedside

To Read Original Article visit:


Comments RSS
  1. multiplex pcr

    Thats great job keep it write the information like CR-based pathogen detection quickly reveals occult blood stream infections in these high-risk patient.It is very helpful and important for us.Thank you very much.


  2. TGI

    Dr. Larry,

    A general question:

    What are the advantages or otherwise in donating blood periodically, lets say once in 3-6 months.?


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