Effects of copper nanoparticles on cerebral endothelial cells: A size dependent activity – proliferation at low concentrations and neurotoxicity at high concentrations.
Effects of copper nanoparticles on rat cerebral microvessel endothelial cells.
The purpose of the current study was to determine whether copper nanoparticles (Cu-NPs) can induce the release of proinflammatory mediators that influence the restrictive characteristics of the blood–brain barrier.
Material & methods:
// Confluent rat brain microvessel endothelial cells (rBMECs) were treated with well-characterized Cu-NPs (40 or 60 nm).
// Cytotoxicity of the Cu-NPs was evaluated by cell proliferation assay (1.5–50 µg/ml).
// The extracellular concentrations of proinflammatory mediators (IL-1β, IL-2, TNF-α and prostaglandin E2) were evaluated by ELISA.
The exposure of Cu-NPs at low concentrations increases cellular proliferation of rBMECs, by contrast, high concentrations induce toxicity.
Prostaglandin E2 release was significantly increased (threefold; 8 h) for Cu-NPs (40 and 60 nm).
The extracellular levels of both TNF-α and IL-1β were significantly elevated following exposure to Cu-NPs. The P-apparent ratio, as an indicator of increased permeability of rBMEC was approximately twofold for Cu-NPs (40 and 60 nm).
Conclusion: These data suggest that
Cu-NPs can induce rBMEC, proliferation at low concentrations and/or induce blood–brain barrier toxicity and potential neurotoxicity at high concentrations.
Related Articles from TGI
Diet may ultimately play an important role in striking the right balance between too little and too much copper consumption
Copper is an integral component of cuproproteins required for many physiological functions. The mineral plays an important and beneficial role in nerve conduction, bone growth, the formation of connective tissue and hormone secretion. However, the recent study published in the journal Proceedings of the National Academy of Sciences (PNAS), suggests that copper appears to trigger the onset and […]
COPPER SURFACES COULD REDUCE HOSPITAL ACQUIRED INFECTIONS In this study, the microbial burden on commonly touched surfaces in the medical intensive care units of three hospitals was determined: 1. to assess the risk from those surfaces, and 2. to determine whether or not copper surfacing would lower that burden, and those risks. Hospital acquired infections […]
Metal oxide nanoparticles (NPs) are among the most highly produced nanomaterials, and have many diverse functions in catalysis, environmental remediation, as sensors, and in the production of personal care products. Nanoparticles have a small aerodynamic diameter. They can easily penetrate lung tissue and cause adverse pulmonary reactions. In this study, the toxicity of several widely […]
Alzheimer’s is a key area of focus for the pharmaceutical industry, but drug development efforts have had little success with many researchers suggesting that by the time patients present with symptoms the disease may be too far progressed to treat effectively. It’s hoped that early detection biomarkers could enable identification of pre-symptomatic Alzheimer’s patients and […]
Curated by: Dr. Venkat S Karra, Ph.D. Metals are an environmental toxin and at the same time they also have therapeutic value, e.g., like the platinum in cisplatin that treats prostate cancer. Also metals such as copper, zinc, and iron are important nutrients -the special properties of these elements that make them useful to living organisms. For example, copper […]
Copper is essential to the proper functioning of organs and metabolic processes and is vital to the health of all living things (humans, plants, animals, and microorganisms). The human body has complex homeostatic mechanisms which attempt to ensure a constant supply of available elements like copper. However, like all essential elements and nutrients, too much or […]
- Neurosurgeon Shows How Low Levels of Radiation Such As Wi-Fi, Smart Meters And Cell Phones Cause The Blood Brain Barrier To Leak (sott.net)
- Copper Oxide Nanoparticles Induce Autophagic Cell Death in A549 Cells (tginnovations.wordpress.com)
- Medtronic Wants Drug Industry Partners for Alzheimer’s – Bloomberg (bloomberg.com)